Biography
- An experienced biophysical and biochemical researcher with a big passion for understanding life with physical principles.
- Currently, working as a PhD candidate in the laboratory of Gijs Wuite at Vrije Universiteit Amsterdam, focusing on DNA-protein interaction with single-molecule manipulation & visualization tools.
- Hold a Master of Science (M.Sc.) Degree from University of Chinese Academy of Science, focusing on the long-acting strategy of biopharmaceuticals, e.g.: PEGylation or albumin-binding domain fused protein.
Interests
- Single Molecule Biophysics
- DNA-Protein Interaction
- Single-molecule Visua- & Manipulation
Education
PhD in BioPhysics, 2022(expected)
Vrije Universiteit Amsterdam
Msc in Biochemical Engineering, 2017
University of Chinese Academy of Sciences
BSc in Food Science, 2014
Northeast Agricultural University
Experience
PhD in Biophysics
PhD Projects include:
- Grab, manipulate and watch single DNA molecule replication
- Direct watching DNAp removes SSB during replication
- Single-molecule studies of the effect of Mg2+on replication and exonuclease activity by DNA
- Quantifying how protein affects DNA melting within replisome under tension
Master Student in Biochemical Engineering
Msc. Projects include:
- Development of thiol-specific PEGylation reagents aiming for elongating protein half-life based on catechol-derivatives.
- Purification and characterization of a long-acting ciliary neurotrophic factor via genetically fused with an albumin-binding domain
Projects
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Ciliary neurotrophic factor (CNTF) is a promising candidate for the
treatment of neurodegenerative or metabolic diseases, but suffers rapid
clearance in body. Here in this project, we constructed a new long-acting recombinant human
CNTF (rhCNTF) by genetic fusion with an albumin-binding domain (ABD) through a
flexible peptide linker, hoping to endow the new molecule prolonged serum
circulation time by binding with endogenous human serum albumin (HSA) and then
utilizing the naturally long-half-life property of HSA.
In this project, we described a new thiol-specific PEGylation strategy based on catechol-derived reactive quinone species. Catechol-derived polyethylene glycol (PEG) was synthesized by coupling linear PEG N-hydroxysuccinimide to dopamine and then oxidized to quinone. PEG-dopaquinone (PEG-DAQ) mostly modifying the free cysteines of two model proteins of truncated flagellin (CBLB502) and recombined human ciliary neurotrophic factor (rhCNTF) evidenced its thiol-specificity.
We develop a method to fast sequence a single DNA molecule, which can be further used for virus detection.
We used a machanical model to quantify how protein affects DNA melting within replisome under tension.
Transitionally exposed ssDNA during replication are coated
and stabilized by single-stranded DNA binding protein (SSB) within the
replisome. How these SSB protein regulates and are displaced by DNA polymerase
(DNAp) however are still poorly understood. Here T7 DNAp and T7 gp2.5 (SSB)
are used as model proteins to investigate their interaction.
Recent Publications
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(2017).
Purification and characterization of a long-acting ciliary neurotrophic factor via genetically fused with an albumin-binding domain.
Protein Expression and Purification.
(2017).
Oxidized catechol-derived poly (ethylene glycol) for thiol-specific conjugation.
Reactive and Functional Polymers.
(2017).
A kind of albumin binding peptide-human ciliary nerve nutrition factor fusion protein and its preparation method and application/一种白蛋白结合肽-人睫状神经营养因子融合蛋白及其制备方法和应用.
Issued on China Patent.
(2017).
A Polyethylene Glycol Modification Reagents and its Preparation and Application/ 一种含有邻苯二醌类官能团的聚乙二醇修饰剂及其制备方法和用途.
Issued on China Patent.
Contact
- longfu2.xu#at#gmail.com
- +31-2059 89284
- De Boelelaan 1081, 1081 HV Amsterdam
- Weekday 9:00 to 17:00